Eur J Cancer 2011 Oct;47 (15): 2299-305. [IF:4.944]
Overexpression of matrix metalloproteinase-12 (MMP-12) correlates with poor prognosis of hepatocellular carcinoma.
Ng KT , Qi X , Kong KL , Cheung BY , Lo CM , Poon RT , Fan ST , Man K .
Department of Surgery and Centre for Cancer Research, LKS Faculty of Medicine, The University of Hong Kong, China.
香港大学李嘉诚医学院外科和癌症研究中心
Abstract
Tumour recurrence and metastasis are pressing issues of hepatocellular carcinoma (HCC) patients who receive surgical treatments. Matrix metalloproteinase-12 (MMP-12), previously identified from our animal model, is involved in tumour invasiveness of rat hepatoma. We aimed to investigate the significance and prognostic value of MMP-12 expression in human HCC. MMP-12 mRNA level of 139 pairs of tumour and non-tumour liver tissues of HCC patients after hepatectomy were investigated by quantitative real-time RT-PCR. MMP-12 mRNA was significantly elevated in tumour liver tissues of HCC patients compared to non-tumour and normal liver tissues. By comparing paired tumour and non-tumour liver tissues, MMP-12 mRNA was overexpressed in 58% of tumour tissue of HCC patients. Overexpression of MMP-12 mRNA was significantly correlated with presence of venous infiltration (p=0.004), high serum AFP level (p=0.012), early tumour recurrence (p=0.018) and poor overall survival (p=0.02) of HCC patients. Moreover, MMP-12 mRNA was an independent factor in predicting the 1- and 3-year overall survival of HCC patients after hepatectomy. Our data demonstrated that MMP-12 mRNA may be a valuable prognostic marker for both overall survival and tumour recurrence of HCC patients after liver resection.
摘要
肿瘤的复发和转移是肝细胞癌手术患者的迫切问题。以前从我们的动物模型中鉴定的基质金属蛋白酶12参与鼠肝细胞瘤的侵袭力。我们的目的是调查人类肝细胞癌MMP12表达的意义和预后价值。通过定量实时RT-PCR技术，肝癌术后的139对肿瘤和非肿瘤肝组织MMP12 mRNA水平被调查。肝细胞癌患者的瘤组织与非瘤和正常组织比较，瘤组织的MMP12 mRNA显著升高。通过比较成对的瘤与非瘤组织，MMP12 mRNA在58%的肝细胞癌瘤组织中被过度表达。MMP12 mRNA的过表达显著相关于静脉渗透的存在（P=0.004），高的血清AFP水平（P=0.012），早期的肿瘤复发（P=0.018），肝细胞癌差的幸存率（P=0.02）再者，MMP12 mRNA是肝癌术后1年和3年幸存率的独立预测因素。我们的数据显示MMP12 mRNA也许是肝癌术后幸存和复发的有价值的预测指标。
